抄録
Some substrates for estimation of serum cholinesterase were synthesized from the derivative compounds of benzoic acid and naphthoic acid.
3, 4-dihydroxybenzoyl choline was specific for pseudo-cholinesterase activity and was hardly hydrolyzed in buffer solution. The disappearance of substrate was measeured by ultraviolet spectrophotometry at 340 nm. Both Within-run and day-to-day imprecision were between 0.6 and 1.4%. Addittion of 18 substances to sera did not almost effect the determination of pseudo-cholinesterase activity.
A good correlation was observed between the present method and the Kalow's method using benzoylcholine as substrate.
