Rapid Assay of Lactate Dehydrogenase in Serum bythe Stopped-Flow Method
1988 Volume 17 Issue 3 Pages 136-141
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1988 Volume 17 Issue 3 Pages 136-141
The stopped-flow method was applied to the emergent or urgent assay of lactate dehydrogenase in human serum by using a novel apparatus for microanalysis, which has newly been designed by one of the authors. The enzyme activity was easily estimated from the initial slope (velocity) of the reaction curve recorded at 340nm for only 5 s. Calibration curve was linear in the range of zero to 1000 IU/I. when 10 μl of serum was used, the detection limit was 30 IU/I and was comparable to that of conventional UV method. The coefficient of variation in within-run and that in between-run were <5% and <7% for the serums with the enzyme activity of >200 IU/I, respectively. Close correlation was found between the present method and the currently available UV method (r=0.988).
