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Volume 17, Issue 3
Displaying 1-7 of 7 articles from this issue
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MARIKO MIYAKE, SHIRO IKAWA, TETSUO MURA, MASAHIDE IKEGUCHI1988 Volume 17 Issue 3 Pages 99-106
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA simple and sensitive enzymatic fluorometric microassay is described for the determination of 3-keto bile acids using 3-ketosteroid-Δ4-dehydrogenase (Δ4 DH) and resazurin.
The present microassay showed high substrate specificity of free and conjugated 3-keto bile acids and the linearity of fluorescence intensity (Emission; 580nm, Excitation; 560nm) to 3-keto bile acids in the range of 2.5 to 80μM with coefficient variation of 2.1% to 3.4%.
The present method was applied to determination of fecal 3-keto bile acids from controls and patients with ileocecal resection or colon resection.
The correlation between HPLC (y) and the present method (X) for determination of fecal 3-keto bile acids was r=0.896 (n=43), and the regression curve was y=0.784x+0.199.
Feces from controls and patients with ileocecal resection or colon resection contained 3-keto bile acids by 35.1±9.1%, 20.5±4.6% and 11.7%±2.5% of total fecal bile acids pergram of dry feces, respectively.View full abstractDownload PDF (839K) -
MUNETADA OIMOMI, SHOGO MASUTA, NAOYA IGAKI, TSUNEO NAKAMICHI, YUICHIRO ...1988 Volume 17 Issue 3 Pages 107-110
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe investigated the problems associated with the assay of fructosamine as an indicator of blood glucose control. In light of the relationships among fasting blood glucose, levels of hemoglobin A1c, and serum fructosamine, this assay appears to be clinically applicable for use as an indicator of blood glucose control during the two weeks prior to the assay. When blood glucose control is undertaken and serum fructosamine is used as an indicator, a serum fructosamine level of 3.1mmol/L is considered to an appropriate target level, except in patients with unstable diabetes, as compared with 2.56±0.30 mmol/L in healthy subjects.View full abstractDownload PDF (449K) -
SHUICHI SAHEKI, AKIRA TAKEDA, KENJI TOKUNAGA, MITSUHARU MURASE, NOZOMU ...1988 Volume 17 Issue 3 Pages 111-120
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSSerum high density lipoprotein (HDL) a nd amyloid protein A (SAA) i n HDLw ere analyzed using ultracentrifugation and isoelectrofocusing in patients with high C-reactive protein (CRP) levels but no serious hepatic and renal insufficiencies, malnutritions, endocrine disorders and hyperlipidemias.
It was indicated that SAA percent of the total HDL proteins or the estimated SAA concentrations in HDL were well correlated with CRP concentrations in various kinds of diseases at acute phase.
The serum HDL and their apo A and C protein concentrations were lower in the CRPpositive patients than in the CRP-negatives, but apo E protein concentration of the former was not different from that of the latter. Although apo A-I /A-II and A-I /C ratios were identical in both groups, A-I /E ratio was low in the SAA-rich HDL. It suggests that such HDL is relatively rich in apo E protein. However, the protein-lipid compositions in HDL were not different significantly between the two groups.View full abstractDownload PDF (4455K) -
MUNETADA OIMOMI, NAOYA IGAKI, SHOGO MASUTA, FUMIHIKO HATA, YUICHIRO MA ...1988 Volume 17 Issue 3 Pages 121-124
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSSince lens protein is a long-lived protein, the levels of advanced-stage products of the Maillard reaction are considered to reflect the sum of accumulated glycation. We nvestigated the relationship between the level of advanced product in cataractous lens nucleus and the motor nerve conduction velocity (MCV) of the peroneal nerve, and the sensory nerve conduction velocity (SCV) of the sural nerve, in diabetic patients. There were significant correlations between the product level and both of these parameters. However, there was no significant relationship between the levels of fasting plasma glucose and hemoglobin Alc immediately prior to surgery and the MCV and the SCV.
These results suggest that glycation measured by advanced Maillard product plays an important role in the etiology of diabetic neuropathy.View full abstractDownload PDF (370K) -
MUNETADA OIMOMI, NAOYA IGAKI, TSUYOSHI OHARA, MAKOTO SAKAI, TSUNEO NAK ...1988 Volume 17 Issue 3 Pages 125-127
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESS3-Deoxyglucosone, which is formed as an intermediate compound in the Maillard reaction, acts on lysozyme to increase its fluorescence and to increase the level of peak L1 compound which we identified previously as an advanced Maillard product. This finding suggests the possibility that 3-deoxyglucosone may act on various types of protein in the body to accelerate the production of advanced Maillard products.View full abstractDownload PDF (339K) -
Studies on Screening Test for Urine of Workers Exposed to Organic Solvent Products Such as Thinners.KIKUEKO KISHINAMI, YASUNORI ARAKIDA1988 Volume 17 Issue 3 Pages 128-135
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe describe a high performance Liquid chromatographic method for the screening test of hippuric acid and o-, m-and p-methylhippuric acids in urine, metabolites of toluene, o-, m- and p-xylenes, respectively. The method has simple, specific and high sensitivity. All the Metabolites were clearly separated in a short time. Methylhippuric acids could not be detected in the normal urine by this method, even if it contains high concentration of hippuric acid. The present method can be used for the health control of workers exposed to organic solvent products such as thinners.View full abstractDownload PDF (761K) -
KEN-ICHIRO KANAYA, KEITARO HIROMI, MASAYUKI TOTANI, TAKASHI MURACHI1988 Volume 17 Issue 3 Pages 136-141
Published: December 30, 1988
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe stopped-flow method was applied to the emergent or urgent assay of lactate dehydrogenase in human serum by using a novel apparatus for microanalysis, which has newly been designed by one of the authors. The enzyme activity was easily estimated from the initial slope (velocity) of the reaction curve recorded at 340nm for only 5 s. Calibration curve was linear in the range of zero to 1000 IU/I. when 10 μl of serum was used, the detection limit was 30 IU/I and was comparable to that of conventional UV method. The coefficient of variation in within-run and that in between-run were <5% and <7% for the serums with the enzyme activity of >200 IU/I, respectively. Close correlation was found between the present method and the currently available UV method (r=0.988).View full abstractDownload PDF (613K)
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