ヒト血清中膵Elastase 1のEnzyme lmmunoassay

抄録

Monoclonal antibodies were raised against human pancreatic elastase 1 and used for a one step enzyme immunoassay. The α₁antitrypsin-elastase 1 complex was employed as the standard in the assay. The assay's measurable range was 1.0-200ng/ml and the intra-assay coefficient of variation was 2.4-4.7%. Analytical recovery of the assay was 95.2±3.86%(x±SD). The average nomal serum level determined by the proposed method was 7.5ng/ml with a range of 2.9-19.2ng/ml. The enzyme immunoassay offers simple. rapid and specific analysis of serum pancreatic elastase 1 for clinical diagnosis.