1990 Volume 19 Issue 4 Pages 354-359
An automated column-switching high-per formance liquid chromatographic method with ultraviolet detection is described for rapid and sensitive determination of the concentration of etizo- lam, an anti-anxiety drug, in human serum or plasma. Samples were subjected to column-switching high-performance liquid chromatography using a short precolumn packed with a bovine serum albumin-coated ODS for sample clean-up and a reversed phase column (TSK gel ODS-80TM) for analytical separation. The detection limit of etizolam is 0.7 ng/ml serum. The recovery of etizolam added to serum (9.8-39.4 ng/ml) was approximately 100% with a standard deviation of 3% or less.
