抄録
A chemiluminescent enzyme immunoassay (CLEIA) was developed for measurina thyroglobulin in serum. This assay is rapid, easy to perform, and highly sensitive. A microplate was used as the solid phase, peroxidase as the labeling enzyme, and luminol and enhancer as the chemiluminescence reagents. The minimum concentration of thyroglobulin detectable by this CLEIA was 0.05 ng/ml. The results obtained with the CLEIA were in good agreement with those obtained by the standard RIA, the correlation coefficients being r=0.98, Y=0.97X-5.1. where Y is the CLEIA value and X the RIA value. The CLEIA was used to measure thyroglobulin serum levels as a means to monitor thyroid cancer. In most patients, the high preoperative levels dropped to nearly 0 ng/ml after total thyroidectomy, and then increased again upon recurrence. In conclusion, measurement of the thyroglobulin serum level changes by this highly sensitive assay is effective for early discovery of thyroid cancer recurrence postoperatively.
