臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
One Component of non Hemoglobin Proteins in the Human Erythrocytes. Isolation and Identification.
西部 ひな
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1976 年 5 巻 1 号 p. 74-78

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one component of molecular weight 30,000 in the supernatant on 65% ammonium sulfate fractionation of the hemolysate was isolated by the method of Sephadex G-75 and CM-cellulose column chromatography, and electrofocusing.
The physicochemical properties of this protein were as follows; molecular weight 30,000±3,000, isoelectric point pH 6.0. Amino acid analysis was performed. lmmunochemical reaction with arlti human carbonic anhydrase B serum was observed. From the above results, this protein was estimated as carbonic anhydrase A.
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© Japan Society of Clinical Chemistry
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