抄録
A new substrate, o-toluoylcholine, was synthesized for the estimation of serum cholinesterase activity. It was specific for pseudo-cholinesterase activity and was stable for storage in cold after dissolving in buffer and against temperature and pH. Serum cholinesterase activity was also able to be estimated by the reaction rate assay using o-toluoylcholine as asubstrate.
o-Toluoylcholine was slightly hydrolyzed by α-amylase, cholesterol esterase and trypsin from the pancreas, as well as by carboxylesterase from the liver.
Good correlation was observed between this enzymatic metho dusing o-toluoylcholine as a substrate and the ΔpH method using acetylcholine as a substrate.
