抄録
Recovery rates for guanidino compound analyses were measured in ten laboratories. Authentic guanidino compounds were added to plasma, and plasma protein was removed by the ultrafiltration using Amicon Centriflo Membrane Cone Type CF 25 (Amicon Corp.). Guanidino compound analyses were performed by the guanidino compound autoanalyzer (model G-520 and GCA-101: Japan Spectroscpoic Co., Ltd. ; model JLC-6AH: Japan Electron Optics Lab. Co., Ltd. ; and other special types). When the samples were adjusted to pH 2-4, the recovery rate for each guanidino compound in plasma was rather high and stable. On the con trary, the recovery rates for taurocyamine, guanidinosuccinic acid, guanidinoacetic acid and β-guanidinopropionic acid were rather lower, when the pH of samples were not adjusted. Therefore the samples should be adjusted to acidic before ultrafiltration, for avoiding adsorption of guanidino compound on an ultrafiltration membrane.
Guanidino compound analyses of ECUM solution and plasma were carried out on the same samples in twelve laboratories. Guanidino compound analyses were performed by several kinds of different methods depend on liquid chromatography and fluoremetry. As the results of the experiment, we found that the variance of measured values by the differen laboratories was very large. This variance may be mainly caused on pretreatment for analysis.
