Abstract We measured bite force in 3,017 children (1,556 boys, 1,461 girls) who performed the new physical fitness test from 1999 to 2006, to analyze the relationship between bite force and the new physical fitness. The results obtained were as follows: 1. There was a strong positive correlation between bite force and increased age in both sexes. 2. Linear regression analysis revealed that bite force, tended to decrease with each generation. 3. Stepwise multiple regression analysis revealed a strong positive correlation between bite force grip strength tested by the new physical fitness tests.