Equilibrium Research
Online ISSN : 1882-577X
Print ISSN : 0385-5716
ISSN-L : 0385-5716
顕微内視鏡による脳深部神経回路のin vivoでの機能解析
船曳 和雄
ジャーナル フリー

2011 年 70 巻 2 号 p. 110-114


In vivo visualization of neural circuits located in the deep brain area with a micro-endoscope
Recent advance in molecular biology enabled us to label specific sets of neurons with several fluorescence proteins. To visualize these labeled cells in vivo, we developed a micro-endoscope system which can be inserted into the brain and can provide circuit images with cellular resolution. The micro-endoscope was made from a commercially available image fiber in which three to six thousand optical fibers are bundled. For functional imaging of neurons, we combined this endoscope with a custom-built confocal laser scanner fitted with a high-speed resonance galvomotor, and obtained confocal endoscopic images at a frame rate of 18-100 fps depending on the number of vertical lines. Our endoscope system visualized fluorescence labeled individual neuron, Ca response in vivo and provided stable images of the neural circuits even in head-restricted alert mice.

© 2011 一般社団法人 日本めまい平衡医学会
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