抄録
Endolymphatic hydrops may be involved in the pathogenesis of Meniere's disease. However, the etiology remains to be clarified. Magnetic resonance imaging (MRI) facilitates visualization of the inner ear structure, and is useful for physiological and pharmacological evaluation. Recently, MRI of the fine structures of inner ears has been performed in humans, guinea pigs, and mice. In this study, we conducted high-magnetic-field MRI in mice to visualize endolymphatic hydrops.
An endolymphatic hydrop model, PhexHyp-Duk/Y mice, was used, as well as a 7.05T MRI device (AVANCE III, 300 wb, ParaVison 5.1 PL2, Bruker GmbH). After intraperitoneal anesthesia, gadolinium contrast medium diluted with physiological saline at a ratio of 8 was administered into the tympanic cavity.
Under mask anesthesia with sevoflurane, the body temperature was maintained by warm water (38°C) circulation. A 9-mm surface coil (Doty Co., Ltd.) was established in the external auditory canal. Using the 7.05T MRI device, inner ear imaging was performed. In the PhexHyp-Duk/Y mice, the presence of endolymphatic hydrops was confirmed.
