抄録
Rabbit antiserum against medaka egg yolk extract was obtained. Vitellogenin (VTG) of medaka was purified from ascites of Erexposed male medaka. Immuno-character was tested by double diffusion analysis and immuno-dot analysis. The antiserum obtained exhibited an immune-reaction with blood and ascites of Erexposed medaka (male), purified medaka VTG, and egg yolk extracts, blood and hepatocyte extracts of female medaka, but not with blood and hepatocyte extracts of male medaka or carp VTG. In double diffusion analysis, two kinds of protein in the ascites reacted to the antiserum. A few proteins other than VTG were also observed by native PAGE. The results suggest that the protein induced by E2 is not only VTG. The antiserum obtained reacted to blood of male medaka exposed to E2,p-nonylphenol or bisphenol-A. Those results were suggested that the antiserum is useful for investigating the influences of xeno-estrogens on medaka.
