魚類由来浮遊培養細胞を用いたゴルフ場農薬の毒性評価

抄録

Cytotoxicity of 19 pesticides used in golf links to two suspension-cultured cell lines from fish (CHSE-sp cell line and FHM-sp cell line) was determined by neutral red assay. The details of the pesticides were 7 insecticides, 7 fungicides and 5 herbicides. Oxine-copper, Chlorothalonil, Thiuram and Captan (they are fungicides), and Chlorpyrifos (insecticide) were highly toxic to both cell lines. It is well known that these pesticides are also highly toxic to carp and water flea. There was good correlation (r > 0.8) between 24-h NR₅₀ to both cell lines and 96-h LC₅₀ to rainbow trout fry and 48-h LC₅₀ to carp of the pesticides used in this study. There was also good correlation (r=0.981) between 24-h NR₅₀ to the CHSE-sp cell line and the FHM-sp cell line. The ratios of NR₅₀ to LC₅₀ (NR₅₀/LC₅₀) of pesticides tested were more than 1. Sensitivity of in vitro test using suspension-cultured cell lines from fish to pesticides was lower than that of in vivo test using whole fish body. However, the suspension-cultured cells can be inoculated directly to the microplate well and the correlation between in vitro test using these suspension-cultured cells and in vivo test using whole fish body was relatively high. Therefore, the suspension-cultured cell lines derived from fish might be useful screening tools prior to in vivo testing of pesticides used in golf links.