Japanese Journal of Food Microbiology
Online ISSN : 1882-5982
Print ISSN : 1340-8267
Original
Studies on a Rapid Method for Detecting Shiga Toxin-Producing Escherichia coli (STEC) in Food Using the QUALIBAXTM Q7 System
Tetsuya MORIMasumi HATAAkimasa WATANABEYoshito IWADEShintaro WADAToyohiko NANBAIchiro TOOYAMAShuhei OKUMAKenzo UEHASHIHirotaka KONUMATakeshi ITO
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2014 Volume 31 Issue 4 Pages 194-203

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Abstract

The purpose of this study was to evaluate the QUALIBAXTM Q7 System (BAX system) for detecting non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O103, O111, O121, O145) and STEC O157. The BAX system real-time PCR assay STEC suite was evaluated, including a screening assay for the stx and two panel assays to identify the target serogroups. For STEC O157, BAX system real-time PCR assay E. coli O157 : H7 was used. The BAX system assay was found to detect STEC O26, O103, O111, O121, O145 and O157 in pure culture. Each assay was 100% inclusive for the STEC strains used. And no cross-reactivity was observed. Sensitivity of a screening assay for the stx was ≧1.8×103 CFU/ml in cultures of ground beef or lettuce. E. coli O157 : H7 was detected at a level of ≧1.8×104 CFU/ml. On a collaborative study, the performance of the BAX system assay was compared with that of a culture method for detection of STEC O26, O111 and O157 in ground beef and lettuce. Sensitivity of the BAX system method was better than a culture method. These results suggest that the BAX system can be used as a rapid method for detecting STEC in food samples.

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© 2014 Japanese Society of Food Microbiology
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