Abstract
A method to detect Campylobacter species by culture in a food pathogen enrichment broth followed by tagged cocktail polymerase chain reaction and DNA strip was developed. When C. jejuni was detected, we measured mutation of amino acid position 86 in the GyrA gene, which is involved in quinolone resistance. The quinolone-resistant strains with GyrA mutation and quinolone-sensitive strains were clearly differentiated simply by measuring their Tm values after SYBR green real-time polymerase chain reaction. To detect Campylobacter from the enrichment broth and predict the quinolone resistance, these methods took only 1 hr.
