Comparison of the Commercial Locate Kit with Conventional Culture Method for the Detection of Salmonella in Raw Chicken
1995 Volume 11 Issue 4 Pages 233-236
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1995 Volume 11 Issue 4 Pages 233-236
The commercial ELISA kit (Locate) was compared with a conventional culture method for the detection of Salmonella in foods. The sensitivity of the ELISA kit was detection of 106 cells/ml of S. Enteritidis, S. Tyhimurium and S. Infantis in pure culture. All of the 23 serovars of Salmonella tested gave correctly positive results, while the 7 strains of C. frendii gave correctly negative results.
Raw meat and liquid egg which had been inoculated with low levels of different serovars of Salmonella gave positive results by the kit and culture method. In 50 samples of raw chicken, the correlation between the ELISA kit and culture method was very high, 98.0%. An ELISA false negative result was obtained in one sample. Salmonella recovered from the enrichment broth was not reactive with the ELISA, because the organism was covered with mucoid substance. The ELISA kit was found to be a rapid screening method for the detection of Salmonella in food.
