Abstract
Listeria monocytogenes have been attracting recent attention worldwide as an important causative agent of foodborne infections. We attempted to adapt the PCR method for quick and specific detection of this bacteria from foodstuff, and carried out basic studies on seven PCR primers (C-D, MonoA-B, hly1-2, prfAI-2, LM1-2, SH2A-B and SI3A-B) regarding the detection by specificity and differences in serovars.
The detection of specificity and different serovars against L. monocytogenes were the highest for primer MonoA-B. The detection sensitivities were good for primers prfAI-2, hly1-2 and LM 1-2. As for specificity, cross-reacting with some strains was observed and bands other than the target PCR products were obtained. Non-specific products disappeared and specificity increased with some primers for bands other than the targets by varying the dilution concentration.
