Detection of Erythrocytic Inclusion Body Syndrome (EIBS) Virus (Piscine orthoreovirus 2) from Ovarian Fluid of Coho Salmon Survivor

Akira Kumagai; Tomokazu Takano; Tomomasa Matsuyama; Takamitsu Sakai; Yuki Nochiri; Ryo Honda; Takumi Kikuta; Miho Honjo

doi:10.3147/jsfp.54.20

短報

Detection of Erythrocytic Inclusion Body Syndrome (EIBS) Virus (Piscine orthoreovirus 2) from Ovarian Fluid of Coho Salmon Survivor

Akira Kumagai, Tomokazu Takano, Tomomasa Matsuyama, Takamitsu Sakai, Yuki Nochiri, Ryo Honda, Takumi Kikuta, Miho Honjo

著者情報

キーワード: erythrocytic inclusion body syndrome, piscine orthoreovirus 2, ovarian fluid, coho salmon

ジャーナルフリー

2019 年 54 巻 1 号 p. 20-23

DOI https://doi.org/10.3147/jsfp.54.20

詳細

抄録

Mortality of farmed coho salmon Oncorhynchus kisutch due to erythrocytic inclusion body syndrome (EIBS) has been posing severe economic losses in Japan since 1986. The etiologic agent of EIBS was reported to be piscine orthoreovirus 2 (PRV-2). We detected PRV-2 in the ovarian fluid samples of 10% of EIBS-experienced spawners (n = 30) by RT-PCR. When each of 30 individual ovarian fluid samples was inoculated into a naïve coho salmon, 37% of the blood samples from the fish tested positive for PRV-2 at 20 days post injection. These observations suggest that the ovarian fluid of EIBS-experienced fish can be contaminated with the infective viral particles of PRV-2.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）