Effects of Hydrogen Peroxide on Egg Laying and Hatching of the Skin Monogenean Neobenedenia girellae

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The effects of concentrations and exposure durations of H₂O₂ on the egg laying and egg hatching of Neobenedenia girellae were investigated. ​Four groups of eight adults or approximately 250 eggs were treated with H₂O₂ as follows: 0 ppm for 30 min (control), 300 ppm for 3 min; 75 ppm for 30 min; 100 ppm for 30 min. ​The adults in the H₂O₂ exposed groups produced significantly fewer number of normal eggs. ​Additionally, the H₂O₂ exposures to the eggs significantly reduced the hatching success of the eggs at < 24 h after laying, but the eggs at 48–71 h after laying were not affected.