Development of New PCR and Quantitative PCR Protocols for the Detection of Plecoglossus altivelis Poxvirus-like Virus in Atypical Cellular Gill Disease of Ayu

Tomoki Koyama; Daiki Komatsu; Tsubasa Uchino; Yuki Midorikawa; Goshi Kato; Takanori Ishikawa; Tomohiro Nishimura; Korenori Takeda; Hideo Fukuda; Shinpei Wada; Motohiko Sano

doi:10.3147/jsfp.55.84

短報

Development of New PCR and Quantitative PCR Protocols for the Detection of Plecoglossus altivelis Poxvirus-like Virus in Atypical Cellular Gill Disease of Ayu

Tomoki Koyama, Daiki Komatsu, Tsubasa Uchino, Yuki Midorikawa, Goshi Kato, Takanori Ishikawa, Tomohiro Nishimura, Korenori Takeda, Hideo Fukuda, Shinpei Wada, Motohiko Sano

著者情報

Tomoki Koyama
Tokyo University of Marine Science and Technology
Daiki Komatsu
Tokyo University of Marine Science and Technology
Tsubasa Uchino
Tokyo University of Marine Science and Technology
Aquaculture Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency
Yuki Midorikawa
Tokyo University of Marine Science and Technology
Goshi Kato
Tokyo University of Marine Science and Technology
Takanori Ishikawa
Tochigi Prefectural Fisheries Experimental Station
Tomohiro Nishimura
Tochigi Prefectural Fisheries Experimental Station
Korenori Takeda
Tochigi Prefectural Fisheries Experimental Station
Hideo Fukuda
Tokyo University of Marine Science and Technology
Shinpei Wada
Nippon Veterinary and Life Science University
Motohiko Sano
Tokyo University of Marine Science and Technology

キーワード: Plecoglossus altivelis poxvirus-like virus, PCR, qPCR, atypical cellular gill disease, ayu

ジャーナルフリー

2020 年 55 巻 3 号 p. 84-87

DOI https://doi.org/10.3147/jsfp.55.84

詳細

抄録

Plecoglossus altivelis poxvirus-like virus (PaPV) is the presumptive causative agent of atypical cellular gill disease (ACGD), which has caused significant losses in aquaculture of ayu P. altivelis in Japan. In this study, we investigated a new PCR protocol and a quantitative PCR (qPCR) protocol with primers designed from the vaccinia virus A16L homologous gene sequence of PaPV. The new PCR showed a positive reaction for affected fish in all eight ACGD cases tested and no cross-reaction with carp edema virus, and was more sensitive than a PCR with BOKE30 primers. The qPCR quantified around 10⁸–10⁹ virus DNA copies/mg in the diseased fish gills.

責任著者(Corresponding author)

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