Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Selection of Suitable Cell Line for Isolation of the Infectious Hematopoietic Necrosis Virus (IHNV) from Ovarian Fluid of Salmonid fish
Toko YoshinakaMamoru YoshimizuYoshio Ezura
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JOURNAL FREE ACCESS

1997 Volume 32 Issue 2 Pages 75-80

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Abstract
Replicability and yield of 2 strains of IHNV in 9 salmonid cell lines (CHSE-214, KO-6, RTE, RTG-2, RTH, RTT, SEH, STE, RTE-2) and 3 non-salmonid cell lines (FHM, EPC, EPG) were compared at a low M.O.I.of 0.001.Yield of IHNV in RTE-2 derived from rainbow trout embryo was as high as those of RTG-2, EPG, FHM and EPC cell lines. Although EPC and FHM cell lines showed high sensitivity to toxic substances in the ovarian fluid from masu salmon Oncorhychus masou, RTE-2 and RTG-2 showed low susceptibility to these substances.These results indicate that the RTE-2 and RTG-2 cell lines are suitable for the isolation of IHNV from the ovarian fluid.
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© The Japanese Society of Fish Pathology
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