In recent years much attention has been given to the problem of pulmonary disease associated with unclassified mycobacteria. In 1935, Pinner pointed out that chromogenic acid-fast bacilli could be found in various human materials, and emphasized the need for careful study in diagnostic procedures. The authers found out 2 patients who have been admitted to a Tuberculosis Hospital, from whose sputum unclassified mycobacteria were found. In case 1, Rapid grower by Runyon were cultured in the sputum. In case 2, culture study revealed photochromogenic unclassified mycobacteria, and the same bacteria were cultured from the surgical specimen. Susceptibility tests revealed these bacilli to be resistant to Streptomycin, PAS, and Isoniazid.