抄録
It is important to prepare the cell culture devices with functional polymer surfaces. Notch ligand delta-1 is considered as one of the important membrane proteins for self-renewal of hematopoietic stem cells. In this study, a polymer matrix immobilized with Notch ligand delta-1 was prepared by photo-immobilization technique for culture the stem cells. It has been reported that photo-reactive polymers bearing azidophenyl groups could immobilize functional proteins without losing their biological activity. Therefore, a novel photo-reactive phospholipid polymer, 2-methacryloyloxyethyl phosphorylcholine polymer bearing azidophenyl groups, was applied as the matrix to conjugate with Notch ligand delta-1. A leukemia cell line, TMD7, was cultured on the bioconjugated polymer surface. The polymer surface immobilized with Notch ligand delta-1 was recognized by TMD7, and the cells efficiently grow on the phospholipid polar group concentrated surface (PC surface) with protein. It was considered that the PC surface provided a suitable environment around the membrane proteins without denaturation. The photo-reactive phospholipid polymer was expected to constitute on in vitro niche to culture hematopoietic stem cells.
