抄録
We investigated whether signal (s) via CD44 may modulate the adhesive interactions between lymphoid cells and human gingival fibroblasts (HGF) . When OS/37 (anti-CD44 mAb) was added to the culture, the adhesion of freshly-isolated peripheral T cells to HGF was enhanced. Interestingly, the pretreatment of HGF with OS/ 37 enhanced adhesion not only resting T cells but also K562 (erythroleukemia line), which is CD44 negative, to HGF. These results suggest that acting on CD44 on HGF, OS/37 induced increased adhesion between lymphocytes and HGF. In order to clarify the molecule (s) involved in the enhanced cell adhesion by OS/37, a panel of mAbs was prepeared to HGF and lymphoid cells and one clone, 3S-B2, was selected on the basis of its ability to inhibit the enhanced cell adhesion by OS/37. Flow cytometric analysis using transfectants revealed that 3S-B2 recognized CD43 expressed on lymphoid cells. Present study suggests that signal (s) via CD44 on HGF may intracellularly transduce a certain signal to induce the CD43-dependent adhesion pathway between HGF and lymphoid cells.
