抄録
Introduction: Recently, we reported that PAMPS and PDMAAm gels induce the chondrogenic differentiation of ATDC5 cells in vitro in maintenance medium without insulin. We hypothesized that hyaluronic acid (HA) affects the in vitro induction effects of PAMPS and PDMAAm gels on the chondrogenic differentiation of ATDC5 cells, depending on the concentration. The purpose of this study was to test this hypothesis.
Methods: This study consisted of three sub-studies: the first was performed to assess the fundamental effects (without HA) of the PAMPS gel, PDMAAm gel, and polystyrene dish surfaces on the chondrogenic differentiation of ATDC5 cells cultured in maintenance medium and in differentiation medium (containing insulin). The second and third sub-studies were performed to analyze the effects of HA supplementation (three concentrations) of the two media on the chondrogenic differentiation of ATDC5 cells cultured on PAMPS and PDMAAm gels, respectively. At 7 days of culture, the gene expression of cartilage markers was examined using real-time polymerase chain reaction analysis.
Results: On the PAMPS gel with the maintenance medium, HA at concentrations of 0.01 and 0.10 mg/mL significantly increased the expression of Col-2 mRNA and aggrecan mRNA in comparison to those without HA. In the cells cultured in the differentiation medium, HA at a concentration of 0.01 mg/mL significantly increased mRNA expression of Col-2 and aggrecan genes in comparison to those without HA. On the PDMAAm gel with the maintenance medium, HA at a concentration of 1.00 mg/mL significantly reduced the expression of these genes in comparison to those without HA.
Conclusion: Mechanical signals from each hydrogel surface significantly affect the chondrogenic differentiation of ATDC5 cells, and HA affects the mechanical signals from each hydrogel surface, depending on the concentration.
