抄録
In this experiment, the-authors observed the depth of tissue penetration of contact Nd: YAG laser irradiation on the circulatory structure of a rat's tongue and the following healing process using the injection replica scanning electron microscope method. The experimental animal was a 6-week-old, male Wister rat, and the irradiation power was 6W delivered for 1 second. Experimental data was collected directly after irradiation and on the 1st day, 4th, 7th, 10th 14th and 21st.
Directly after irradiation, scanning electron microscopic observation of the tongue's surface showed that the wound was large and that the blood vessels surrounding the wounded area were well-sealed by laser irradiation. There was very little fluid secretion and the blood vessels were well-fused together. On the 4th day, the healing was progressing with great activity, and numerous blood vessels were reaching out from the edge of the wound towards the center. On the 7th day, the blood vessels had joined together and the recovery of the circulatory network of the new tissue was nearly complete. On the 21st day, observations showed that capillary loops directly beneath the filiform papillae were gradually being reconstructed from the edge of the wound.