Abstract

Experimental studies in vitro and vivo were performed to evaluate the effect of PDT using pulsed Nd-YAG LASER with Pheophorbide-A (Ph-A) for human gastric cancer. In vitro study we used three cell lines; KATO III, KKLS, NKPS. The cytocidal effect was evaluated in the MTT assay three days after treatment. In vivo study a human gastric cancer transplanted subcutaneously into the backs of nude mice (BALB/c-nu) was used. We evaluated the pharmacodynamics in two ways of ph-A administration; I. P and I.T injection. We also evaluated the photodynamic effect in I. T. injection of Ph-A. The PDT using Ph-A exerted a synergistic anti-tumor effect against the gastric cancer cells both in vitro and in vivo, But Ph-A was not accumulated to the tumor in the way of I. P. injection, so that suggested the necessity of local administration for the sake of effective PDT.