Photodynamic Therapy with ATX-S10 and Nd: YAG-OPO Laser in Mice

Abstract

Recently, new photosensitizers which have a greater affinity for malignant tissue, longer absorption wavelength bands, and that hold hope for deeper effects by PDT than with HpD or PHE (Photofrin). We report the effect of such a new photosensitizer, ATX-S10 and a new laser equipment, Nd: YAG-OPO laser, which is a tunable laser.
Meth-A sarcomas were transplanted on the back of 20 BALB/C mice. When the tumors growth to 1.5 or 2.0 cm in diameter, we divide the mice into Photofrin injected group and ATX-S10 injected group. Laser equipment for excitation light was Nd: YAG-OPO tunable laser. We chose a wavelength 630 nm to excite Photofrin and 670 nm for ATX-S10. The energy density was constant at 50 joules for cm2. We ecaluate the effect of PDT to measure the depth of the tumor necrosis.
The result of this study shows that ATX-S10 had a deeper effect (more than 5 mm deeper) by PDT than Photofrin.