灌腸に関する研究

Calciumの大腸内吸収と胆汁酸併用の意義に就て

抄録

The following experiments were performed in order to investigate the significance of bile or biliary acid administered as enema in combination with calcium preparations, thereby further to elucidate the mechanism of absorption of calcium from the large intestine. First of all, the methods of administration of biliary acid in combination with calcium preparation were scrutinized, and then the influence of ligature of common hepatic duct and cystic duct on the absorption of calcium from the large intestine. On the other land, the bilifaction by. the rabbit, the common hepatic duct of which was ligatured, was investigated. Further, in order to examine the occasional absorption of calcium from the large intestine even when it was administered alone as enema, the correlationship with the room temperature was also investigated.
1) When calcium preparation alone was administered as enema, the absorption of calcium chloride was influenced by the room temperature most extensively. At the temperature exceeding 25°C., the content of calcium in the serum demonstrated an increase of 16.57 mg/dl at the maximum 210 minutes after the enema. Following the above, calcium salicylate demonstrated an increase of 14.38mg/dl at the maximum at the temperature exceeding 25°C. In the cases of calcium lactate, calcium glycerophosphate, calcium carbonate or calcicol, the absorption was not affected by the room temperature. They were not absorbed when they were administered alone as enema.
2) When calcium chloride alone was adminstered as enema after the subcutaneous injection of taurocholic acid, the absorption of calcium from the large intestine was observed. In the case of calcium salicylate, the absorption was noted on some occasions, but not on others. No absorption was observed in the case of calcium lactate.
3) A similar tendency was observed with the intravenous injection of taurocholic acid. Calcium lactate was not absorbed even at 30°C.
4) When calcium lactate or calcicol combined with taurocholic acid was administered as enema, both of the calcium preparations were absorbed very well.
5) In the cases of calcium lactate or calcicol combined with glycocholic acid, both of the calcium preparations were absorbed. In the case of calcium carbonate, no absorption was observed.
6) The enhancement of calcium absorption from the large intestine by cholic acid was weaker compared with those of taurocholic acid or glycocholic acid.
7) In the cases of calcicol or calcium lactate combined with bile of various animals (ox, pig and rabbit) administered as enema, the calcium content in the serum was increased indicating the presence of the absorption of calcium from the large intestine. In the case with rabbit bile, the enhancement seemed strongest, which might be attributed to the higher room temperature at the time of experiment.
8) When calcium chloride alone was administered as enema after the ligature of the common heptic duct and cystic duct, the increase of serum cak ium was observed indicating the presence of the absorption of calcium from the large intestine. However, no major difference from the control test was noticed in this case. In the cases of calcium carbonate, calcium lactate, calcium glycerophosphate and calcicol, the content of serum calcium remained unchanged. In the case of calcium salicylate, no remarkable difference from the control test was noted.
9) The content of biliary acid in the serum after the ligature of common hepatic duct indicated increment up to the 9th day from the ligature though slight in the amount.
10) When calcium chloride or calcium lactate was administered as enema in combination with rabbit bile, cholic acid or glycocholic acid, the content of biliary acid in the serum demonstrated an increment.
In short, when calcium preparations are to be administered as enema in combination with bile of various animals or biliary acids,