Abstract
Believing that the knowledge in the absorption of histamine in the intestinal tract and in the influence of various chemicals on the absorption of histamine not only play an important role in the investigation of the cause of Ekiri-like disease, ileus etc. but also is very significant to the treatment of these diseases, the author conducted experiments concerning the absorption of histamine in the small intestine, and further investigated the influence of various substances administered in combination with histamine on the accumulation of histamine in the liver after the absorption.
Nakamura's method was employed for the extraction of histamine, and a biological method was used for the quantitative estimation of histamine. For quantitative estimation of blood sugar, Hagedorn-Jensen's method was employed, and for quantitative estimation of sulfonamide, Tsuchiya-Kawamura's modification employing p-dimethyl-aminobenzaldehyde was used. Samples of blood were taken by heart puncture 30 minutes after the drug was introduced. In the cases of animals died within 30 minutes from the introduction of the drug, samples were taken immediately after the death. The liver was taken out immediately after the bleeding by heart puncture. In the case when the animals did not die within 30 minutes from the introduction of the drug, the liver was taken out immediately after the bleeding to death. As to the method of administration of drugs, intraduodenal introduction was generally employed.
The principal results obtained are as follows :
Histamine hydrochloride is absorbed well from the small intestine even by single introduction. However, the absorption is greatly affected by the room temperature. The absorption of histamine is enhanced at a higher room temperature. In the case when the histamine solution is on the acid side, the absorption of histamine in the small intestine is slightly enhanced contrary to the case when the histamine solution is on the alkaline side. Cattle bile or calcium chloride, especially cattle bile, remarkably enhances the absorption of histamine. Glucose or fructose evidently inhibits the accumulation of histamine in the liver.
