Abstract
The author investigated the sulfuric acid conjugation of vanillin, bromovanillin methylprotocatechualbarbituric acid and bromomethylprotocatechualbarbituric acid, in rabbit, and further investigated the influence of the hypnotica (barbital and phenobarbital) on the sulfuric acid conjugation. He also investigated the behavior of barbital and phenobarbital on the glucuronic acid conjugation of bromovanillic acid. From the histological findings of liver of the rabbit treated with the above various substances, the author attempted to learn the extent of the toxicity of vanillin and its derivatives. The followings are the results of the investigation.
Vanillin, bromovanillin and their barbituric acid derivatives conjugate sulfuric acid or glucuronic acid without demonstrating any general symptoms inn the rabbits. However, a minor part of the above substances are excreted as vanillic acid after oxidation. On the other hand, the histological damage in the liver of the rabbits progresses in a relatively mild manner.
