Abstract
The autor conducted histopathological investigations on the influence of single or combined administration of INAH and tibione on mice, primarily on the liver. As experimental animals, mice were employed. In the cases of the single administration of INAH, the doses were 10 mg/kg per orally and 10 mg/kg, 60 mg/kg and 100 mg/kg subcutaneously. As reg-ards the single administration of tibione, 0.1 mg/kg, 0.5 mg/kg, 1.0 mg/kg, 2.5 mg/kg and 5.0 mg/kg per orally and subcutaneously were given. In the cases of combined subcutaneous administrations of INAH and tibione, each of the doses of INAH was combined with 0.1 mg/kg, 0.5 mg/kg, 1.0 mg/kg, 2.5 mg/kg and 5.0 mg/kg of tibione. Similar combinations were made with 10 mg/kg of INAH in the cases of per oral administrations. Daily administrations were conducted for 30 days, and then observed for 1 week before the animals were sacrificed.
In the single administrations of INAH, a correlation (equilibrium) was noted between the increase in the dosis and the decrease in the dosis and the decrease in the body weight. Nevertheless, up to the dosis of 1.0 mg/kg of tibione in the single as well as in the combined administration, the findings were not eminent compared with those in the single administrations of INAH.
In the cases of the single subcutaneous administration of INAH (10 mg/kg, 60 mg/kg and 100 mg/kg), swelling, granular degeneration, vacuolar degeneration, disordered arran-gement, changes in the nucleus, fusing degeneration and necrosis of the hepatic cells observed in the long term group were milder than those observed in the medium term group. Consequently, these changes were conisidered to be inconsistent reciprocal changes.
The findings such as turbidity, swelling of fusing changes in the hepatic cells in the cases of the single subcutaneous administration of tibione were slightly mild up to the dosis of 1.0 mg/kg, but they gradually became stronger up to the dosis of 5.0 mg/kg.
In most cases of the single per oral adiministrations of tibione up to 2.5 mg/kg, the above changes were found mild, and they became slightly stronger at 5.0 mg/kg.n the present experiment, the findings in the hepatic cells such as swelling, granular degeneration or fusing changes due to combined subcutaneous adminstration of INAH and tibione up to the combination with 2.5 mg/kg of tibione were found not much different from those due to the addition of a small amount of tibione. However, by the combination with 5.0 mg/kg of tibione, these changes demonstrated a mild tendency of increase. The changes due to the administration of INAH up to 100 mg/kg were found greatly affected by the combination with tibione.
