Abstract
Rabbits were preliminary blockaded with carbon cinnabar or carmine fluid and inoculated with supernatant fluid obtained by centrifuge 15 minutes at 3, 000 r. p. m. of 1: 10 emulsion of brain taken from the mouse infected with Japanese encephalitis virus (Nakayama strain) and manifesting the symptoms.
Rabbits were inoculated with 0.7 cc of the supernatant intravenously and 0.1 cc of the same fluid into the parenchyma of the brain tissue.
Cerebral changes and changes in kidney were comparedd histopathologically.
Parallel relationship between the degree of infection and histopathological chages in kidney were observed. Although the principal importance should be placed in the degenerative changes in loop epithelium in glomerulus, it was hard to ignore the inflammatory elements such as multinuclear cells, exudation and circulation arrest. In view of the above, the anther included the changes in the category of acute glomerulitis. The decrease or disappearance of the argentafiinity of the fibers of the glomerular and basement membrane of uriniferous tubular was partly attributed to the degeneration of uriniferous tubular epithelium, and partly to the circulation arrest in the interstitium.
Resolution and necrosis of the tubules and glomeruli were observed in 6 cases. These facts give ample proof in considering the route of infection and mechanism of action of toxic substance of Japanese encephalitis.
