E. coli O-23の菌体成分に関する研究

第1報 O物質の精製と構成糖について

抄録

From the significance of antigenic structure and from the important role in infection, many efforts have been done to elucidate the chemical structure of O antigen of gram negative enteric bacteria. After 18 hours' incubation, bacteria were spun down by centrifugation. Zine chloride was added to the supernatant making white precipitate which as followed by washing with distilled water and by elution with disodium-phosphate solution. The crude extract was purified by ammonium sulfate fractionation and zone electrophoresis. Endotoxin thus obtained showed antigenic pyrogenic and lethal effects an experimental animals and precipitated by O antiserum. The chemical component of this toxin is lipidpolysaccharide-protein comple. In this paper the chemical comgonet of polysaccharide was presented. Many papers have been presented about the O antigen of E. coli : NCV 1, 055 NCL, 086, 018, 08, 026, 0111. These results aresummarized in Table 5.
1) The antigenic substance of E. coli O 23 was purified and fhe chemical component of polysaccharide was studied. The chemical components of polysaccharide were glucose, galactose, manose, xylose and glucosamine.
2) The sugar which had large Rf value in paper chromatography, was obtatined. This sugar could not be comparing with abequose and tyvelose, because these sugar were not available as the standared in this moment.