抄録
Histochemical technique for the localization of cholinesterase by Koelle has been modified and improved.
For the specific ChE determination 20% and for the non-specific ChE 24% Na2SO4 were used as storage solutions. Except Mal (NaH maleate: 1.9g, N·NaOH: 10.4ml., q.s 100ml., pH12.6), the same reagents for the incubation solution were used as Koelle's. Optimal pH of the incubation solution for the determination of specific ChE was 6.5-6.7 and for the non-specific ChE was 7.7-7.8. MgCl2 in the incubation media for the non-specific ChE determination was excluded since magnesium ions inhibited non-specific ChE competitively while they activated specific ChE.
