Abstract
Varying kinds of sexual hormones have began to be used extensively with considerable effects for the nursing of immatured babies. In this connection, studies were undertaken with an intention of knowing the influence of those hormones on the animals of embryonic stage by the use of embryonating eggs.
Eggs of white leghorn were incubated at 38°-39.5°C and nonfertile and dead eggs were removed by the examination made on the 5th day of incubation. On incubation, eggs received the injection of hormones of varying concentrations (per 0.1 cc of egg white) as listed below :
Estradiol benzoate (E) 0.1, 0.02, 0.001, 0.002 mg ; Estradiol benzoate 0.02 mg+Progesterone 0.01 mg+vitamin B1 0.5 mg ; Estradiol benzoate 0.002 mg+Progesterone 0.001 mg+vitamin B1 0.05 mg mg (EB) ; Testosteron propionate (T) 0.1 and 0.01 mg; Methylandrostendiol (M) 0.1 and 0.1 mg ; Chorionic gonadotropin+Anterior pituitary gonadotropin (S) 2 and 0.2 rabbit units.
Embryonating eggs were sacrificed on the 15th and 20th days of incubation respectively for the measurements of the body weight of embryos, quantity of the allantoic water and the length of the femur.
Body weight of sequal hormone loaded embryos showed and increase on the 15th day of embryonation except the ones loaded with E 0.002 mg which showed a slight decrease. Especially, remarkable increase of the body weight was observed on the embryos loaded with T 0.01 mg and M 0.1 mg. Embryos loaded with EB also showed a decrease in the body weight.
Eggs on the 20th day of incubation also showed the increase of body weight except those loaded with EB. Especially, the increase of body weight was remarkable on the embryos loaded with E 0.1 mg and 0.002 mg, T 0.005 mg and M 0.01 mg. Embryos loaded with T 0.1 mg, however, showed a moderate decrease of body weight.
As for the femur and she quantity of allantoic water, there was no appreciable difference by he loading of hormones in concentrations specified herein.
