Sinomin, a sulfa drug of durable effect, was administered on various infections of gynecology. Out patients of cystitis, inflammation of the genital organs and mastitis received Sinomin of the initial dose 2.0 g followed by 1.0 g every 12 hours thereafter. Evaluation of the therapeutic effect was done by the finidings of bacteriological tests and the changes observed in the subjective and objective symptoms of the patients.
The drug showed an excellent effect in the treatment of cystitis as well as the inflammation of related organs. It was effective for the treatment of the mastitis on early stage. As for the inflammations of genital organs, those which had been caused by coli bacilli and those with acute symptoms required the combined use of Penigin C suppository. Otherwise, it was not effective.
Administration of Sinomin with long intervals could show therapeutic effects comparable to the administration of other sulfa drugs with shorter intervals. Besides, the period of treatment was shortened by the use of Sinomin.
As for side reactions, urticaria was observed on one of the patients received Sinomin.

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Investigations were made on the effect of “C-Noburon injection”, which is a sedative and antalgic preparation, on the postoperative pain and as basal anesthesia in the field of obsterics and gynecology.
Stated in the following are the results obtained thereof :
1. Basal anesthesia : As basal anesthesia, 2 cc of Noburon was injected intramuscularly 30 minutes in advance on 4 cases who were going to receive operatiods under lumbar anesthesia. This injection was recognized to be quite satisfactory as basal anesthesia, all the patients being deprived of the feeling of anxiety and tension against operation. There was, moreover, no lowering in the blood pressure.
2. Postoperative pain (lumbar anesthesia and Labonal) :
a. Two cases for which the injection of Noburon was made immediately after operation : Both cases were completely free from pain until the following morning. They complained of moderate pain after waking up, but it was of not such an intensity to required the administration of antalgic. There was no abnormality in their blood pressure nor there was any indication of side effects.
b. Nine cases for which the injection of Noburon was made after they complained of postoperative pain : There were 3 patients out of 9 who were free from pain untill the following morning by a single injection of 2 cc. In 1 case, the injection was completely ineffective and necessitated the use of morphine. In the remaining 5 eases, though they complained of pain during the night, an addition injection of 2 cc could deprive them of their pain until the following morning.
3. Pains in the terminal stage of the cancer of uterus : Three patients in the terminal stage of the cancer of uterus with sever lumbago, hypogastric pain and the pain of lower limbs received the injection of Noburon in a dose of 2 cc twice during the night and once during the day time. Two of them responded with excellent analgesic effect. In the remaining case, though the injection was effective temyorarily, it became necessary to use a morphine preparation. In all cases, there was no side effects and the lowering of blood pressure was not recognized in spite of the continuous use of the drug.
4. Anesthesia for endometrial currette and artificial abortion : An intramuscular injection of Noburon 2 cc was given 30 minutes prior to the operation. Except the cases in which the cervix had already been opened like in the case of incomplete abortion, topical anesthesia by the of 1 % procaine hydrochloride was applied simultaneously.
Out of the total 17 cases received the injection, excellent anesthetic effect was recognizeed on 5 cases (29.4%), fairly good effect on 10 cases (58.8%), slightly effective on another and the injection was not effective in the remaining cases. Marked lowering in the blood pressure was recognized in 2 cases. One could restore her normal blood pressure by the injections of Vitacampher 1 cc and Theraptic 3 cc, while the other cases presented mild status of shock because of the bleeding due to abortion. It is not adexuate to attribute the cause of the lowering of blood pressure in those two cases to the side effect of Noburon alone.

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Reports were made on the 2 cases of Naevus comedonicus, an atypical one observed on the upper half of the body of a male patient aged 72 and the other a typical one observed on the left side of the neck of a male patient aged 32.
Varying names have been given denoting the disease under discussion, but the authors recommend to call it as Naevus comedonicus. Histologically, the follicles of hair were found to have markedly opened and contained keratic thrombus. In the former case, cord-like epithelial proliferation was recognized from the follicular wall towards the corium together with a number of naevus cell like cells. The sebaceous gland was found to be either absent or atrophied. In the latter case, cornification of the excretory duct of sebum was remarkable and the sebaceous gland was found to have markedly swollen. It is considered, therefore, that the cornification of the excretory duct of sebum is playing an important role in the development of Naevus comedonicus.

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Examinations were made on the olfactory sense and on both subjective and objective nasal symptoms of women under menstruation, pregnancy and climacterium. Stated in the following are the results obtained thereof.
Varying examinations were made on 30 women under menstruation, but subjective complaints were found in only 4 cases (13 %) . After classifying those women into 3 groups of regular and irregular menses and dysmenrrhea, examinations of olfactory sense were made on different stages of before, during and after respective menstrual period. But, findings of practical importance were not detectable both in nasal symptoms and in the sensibility of the olfactori mucosa.
Olfactory sense examinations were also applied on the cases of hyperemesis and on those in varying stages of pregnancy, but there were on significant findings. Findings of the examibations made on the women in climateric stadium were also the same.
Accordingly, it is believed that the subjective disturbance in olfactory sense of the women under menstruation, pregnancy and climacterium is due to the psychological factors rather than the mechanical changes produced in the olfactory sense

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Varying kinds of sexual hormones have began to be used extensively with considerable effects for the nursing of immatured babies. In this connection, studies were undertaken with an intention of knowing the influence of those hormones on the animals of embryonic stage by the use of embryonating eggs.
Eggs of white leghorn were incubated at 38°-39.5°C and nonfertile and dead eggs were removed by the examination made on the 5th day of incubation. On incubation, eggs received the injection of hormones of varying concentrations (per 0.1 cc of egg white) as listed below :
Estradiol benzoate (E) 0.1, 0.02, 0.001, 0.002 mg ; Estradiol benzoate 0.02 mg+Progesterone 0.01 mg+vitamin B₁ 0.5 mg ; Estradiol benzoate 0.002 mg+Progesterone 0.001 mg+vitamin B₁ 0.05 mg mg (EB) ; Testosteron propionate (T) 0.1 and 0.01 mg; Methylandrostendiol (M) 0.1 and 0.1 mg ; Chorionic gonadotropin+Anterior pituitary gonadotropin (S) 2 and 0.2 rabbit units.
Embryonating eggs were sacrificed on the 15th and 20th days of incubation respectively for the measurements of the body weight of embryos, quantity of the allantoic water and the length of the femur.
Body weight of sequal hormone loaded embryos showed and increase on the 15th day of embryonation except the ones loaded with E 0.002 mg which showed a slight decrease. Especially, remarkable increase of the body weight was observed on the embryos loaded with T 0.01 mg and M 0.1 mg. Embryos loaded with EB also showed a decrease in the body weight.
Eggs on the 20th day of incubation also showed the increase of body weight except those loaded with EB. Especially, the increase of body weight was remarkable on the embryos loaded with E 0.1 mg and 0.002 mg, T 0.005 mg and M 0.01 mg. Embryos loaded with T 0.1 mg, however, showed a moderate decrease of body weight.
As for the femur and she quantity of allantoic water, there was no appreciable difference by he loading of hormones in concentrations specified herein.

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Stated in the following are the findings of the studies made on the tissue-culture of the vesical mucosa of rabbit.
1. Rabbit serum obtained by usual method showed pH of 8.22 ± 0.17 and there was a slight increase by storage on the 7th day.
2. Heparin added plasma of rabbit showed pH of 8.15-8.37 and the same of chicken 8.17-8.41. They showed an increase of 0.12 and 0.27 respectively by storage on the 7th day. Mixture of the two kinds of plasma showed pH of 8.25±0.02.
3. Embryo extract prepared from the eggs of 7-9 days of embryonation by adding an equal amount of Ringer's solution showed pH of 6.61-6.65 without demonstrating any appreciable changes by storage on the 7th day.
4. Culture medium containing Ringers's solution, rabbit serum and embryo extract in a ratio of 2 : 2 : 1 showed pH of 7.69-7.79 without demonstrating appreciable changes for a period of 7 days.
5. Changes observed on pH of the buffered (1/10 N KH₂PO₄, 1/10 N Na₂HPO₄, 1/10 N HCl) Ringer's solution, Tyrode's solution and the culture medium were as shown in Tables 9-17, namely, though there were slight changes on the 3rd day of storage, there were no appreciable changes until cn the 7th day.
6. Culture medium containin 5 % of the buffer solution showed no appreciable changes in pH in spite of the storage at varying temperatures of 20°, 30° and 38°C for a period of 7 days.
7. Influence of the buffer solution on the growth of the vesical mucosa was as follows :
a. When Ringer's solution was used as culture medinm, 11% growth was observed by the addition of 1/10 N KH₂PO₄ to a concentration of 1% and only 1% growth by the addition of 1/10 N Na₂HPO₄ to a concentration of 10%. No growth was rccognized with the buffer of higher concentrations. Addition of 1/10 N HCl completely inhibited the membranous growth of the mucosa.
b. When Tyrode's solution was used, the addition of 1 and 5% of 1/10 N KH₂PO₄ yielded the growth of 11-2% growth respectively, addition of 1, 5 and 10% of Na₂HPO₄ yielded the growth of 12, 5 and 2% and the addition of 1% 1/10 N HCl yielded the growth of 7%.
c. When the mixture of Ringer's solution, rabbit serum and embryo extract was used, the addition of 1, 5 and 10% of 1/10 N KH₂PO₄ yielded the growth of 18, 7 and 1% respectively, the addition of 1, 5 and 10% of 1/10 N Na₂HPO₄ yielded the growth of 16, 9 and 4% and the addition of 1% 1/10 N HCl yielded the growth of 9%. But the addition of buffer in higher concentrations inhibited the membranous growth of the mucosa.
8. Results of the tissue-cultures made by the use of above mentioned 3 kinds of culture media showed that the growth was 32-39% when the mixed culture medium was used, it was 26-28% when Ringer's solution was used and 18-28% when Tyrode's solution was used. Namely, the best growth was observed when the mixed culture medium was used.
9. Growth of the cells in the medium containing buffer was inferior to the one in the medium without and it is considered to be due to the growth inhibitory influence of the buffer.
10. Optimal pH for the growth of the vesical mucosa of rabbit was 7.5-8.1 though it showed proliferation at a vast pH range of 6.0-8.8.

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Employing several kinds of salt solutions and homologous serum, tissue-cultures of the vesical mucosa of rabbit were undertaken. Investigations were made, moreover, on the influence of different temperatures of cultiuation on the growth of vesical mucosa cells. Stated in the following are the results obtained there of :
1. Several culture media (Ringer's solution 4 : serum 4 : embryo extract 2) containing different sera, namaly, the serum of the same rabbit, that of a different rabbit and the pooled sera of several rabbits were prepared for the cultivation of the vesical mucosa of rabbit. Best growth was observed when the medium containing the serum of the same rabbit being 32.4% which was followed by 26.9% of the pooled sera and 21.7% of the serum of a different rabbit.
2. As for the influence of the temperature on the growth of tissue-cultured vesical mucosa, the growth was 32% at 38°C, only 5% at 30°C and no growth was observed at 20°C. Namely, the growth of vesical mucosa was in proportion to the temperature of cultivation.
3. Culture media containing either one of Hanks' solution, Earle's solution or No. 199 in the place of physiological saline gave much inferior growth to that of the medium containing physiological saline and it was concluded that these media are not adequate for the tissue-culture of the vesical mucosa of rabbit.

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Utilizing the action of RNA (ribonucleic acid) and try panblue, studies weres made on the influence of carbohydrates on the hemolysin production of Streptococcus hemolyticus. In the present study, a total of 51 standard and isolated strains of Lanceield's Groups A, B, C, D and G were used.
Antihemoiytic action of carbohydrate appeared to be most evident when the group A strains were used in combination with glucose on the blood agar medium, but this action was not so much evident in the fluid medium and glucose was most effective when applied in its pure form. Production of hemolysin was favoured by the addition of RNA in the case of group A strains, but this action seerned to be inactivated by the presence of glucose. Trypanblue was found to react much more intensely than glucose on the strains other than those of group A. Its antihemolytic action was remarkable even when it was used immediately before titration demonstrating a considerable difference from the influence of glucose.
By the antihemolytic activity on group A strains, carbohydrates were divided into 3 Groups, namely, Group I (glucose, fructose, mannose, maltose, sucrose), Group II (lactose, galactose, mannitol), Group III (rhamnose, raffinose, arabinose, xylose, inulin, sorbitol, trehalose, melibiose) .
Generally, this grouping of carbohydrates was likewise applicable for the other groups of streptococcal strains.
Antihemolytic zone was recognized at low dilutions in hemolysin titration of the fluid culture containing glucose and RNA. It was found, further, that in comparison with that in plain broth culture, the so-called nucleic acid effect on hemolysin production was effected by the presence of glucose in the blood agar broth culture. Differentiation of group A streptococci described by Kobayashi et al can be attained by their hemolytic reactions on the glucose, glucose and RNA, RNA and control blood agar plates more precisely.
To simplily this procedure, so-called PSAD-method (patch on the stab in agar disk) was addopted. Namely, in a set of 4 different combinations, glucose, RNA and control paper patches were placed on a small blood agar disk in a petridish which was stabbed with organismus by the use of needle and incubated for a over night. Patches were removed immediately before reading results.

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Stated in the following are the conclusions drawn from the studies of the influence of Chlorpromazine on the reproductive functions of female animals :
1. Sexual cycle of rats was inhibited by Chlorpromazin and this inhibitory influence parallelled the dose administered. Sexual cycle was restored to normal upon suspending the administration.
2. On mice, the administration of Chlorpromazine during pregnancy scarcely influenced the period of pregnancy and the embryos received little influence, the time of delivery being normal in most cases.
3. Reproductive ability of Chlorpromazine pretreated mice was found to be lowered and the period from mating to the delivery was prolonged. The size of a litter was smaller but the weight of individual suckling was comparable to that of the control. No appreciable difference from the control was observable in the growth of the litter.
4. Ovulation of rabbit by the injection of CuSO₄ 5 mg by two injection method was inhibited by the administration of Chlorpromazine in a dose over 10.4 mg/kg.
5. Pretreatment with Chlorpromazine in a dose of 35-175 mg failed to inhibit the ovulation of rabbit elicited by the administration of Gonadotropin.
6. In either case of in vivo or in vitro experiment, Chlorpromazine lowered the O₂ consumption of the rabbit diencephalon. In vitro, this lowering in O₂ consumption was especially strong in the posterior part of the diencephalon. It was rare, however, to lower the O₂ consumption of cerebral cortex, cerebellum, hypophysial anterior lobe and the ovary.
In view of the findings of experiments stated above, it was concluded that Chlorpromazine inhibits the functions of diencephalon, especially hypothalamus, disturbs the functions of the diencephalon-hypopheseal anterior lobe-sexual gland system inviting the lowering of the reproductive functions of female animals.

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