Abstract
Varying dilutions of ectromelia virus were inoculated into the chorioallantoic cavity (first experiment) and amnionic cavity of the embryonating eggs. By successive passage in the former and by the difference in the dilution of virus inoculated in the latter, biological observations were made on the development of eggs as well as on the mice inoculated with varying test materials. At the same time, moreover, histopathological investigations were made on the changes of various egg membranes (allantoic membrane, amnionic membrane and inner shell membrane) produced at the site of inoculation and those of the different locality as well as the changes by the difference in the concentration of virus inoculated.
In both the first and the second experiments, macroscopic changes were severer, mortality rate was higher and the survival period was shorter in the earlier stage of transplantation and with higher concentration of the inoculum used. As for the histological findings of the shell membrane, the eggs on earlier stage of transplantation and those received the inoculum of higher concentration showed stronger degenerative changes with only moderate hydropic changes. While, the eggs on the later stage of transplantion and those received the inoculum of lower concentrationn showed much weaker degenerative changes with advanced hydropic changes in both experiments.
It isconsidered that both the difference in the amount of virus inoculated and the number of transplantations repeated are influencing each other on the multiplication of virus as well as the destruction of the metabolic processes of normal cells by the toxic effect of the virus and the disturbance of blood circulation producing the changes stated above.
