抄録
Stripping of the horny layer and the epidermis was given on the skin of both rabbits and human beings and skin samples were collected with certain definite intervals for a period of 30 days in order to investigate the changes produced in the contents of SH-group, SS-group, nucleic acids aed succinic dehydrogenase employing the direct (d-) and indirect (i-) methods of nitroprusside reaction (N. P.), DDD method (Barrnett-Seligman), Bennett method, nucleic acid staining and neotetrazolium method (NT) . Stated in the following are the results obtained thereof :
1. Processes of the traumatic healing were divided into the following 6 stages by the characteristic changes observed on the contents of the substances stated above.
Stage I : For 3 hours after stripping (so-called shock stage), various staining reactions remain either unchanged or weakened.
Stage II : From 3 hours after stripping to 12 hours (hypertrophy stage of the basal and the prickle cells), sudden increase in SH-group and the increase in ribonucleic acid and succinic dehydrogenase in the latter half of this stage by d-N. P, method.
Stage III : From 12 hours after stripping to 5-7 days (stage of cell division), SH-group and ribonucleic acid show the strongest colour reaction by d-N. P, method.
Stage IV : From 5-7 days after stripping to 20 days (stage beginning the formation of horny layer), SH-group, succinic acid dehydrogenase and desoxyribonucleic acid show the strongest colour reaction by Bennett method, and SH-group and SS-group show the strongest colour reaction by DDD method in the latter half of this stage.
Stage V : From 20 days after stripping to 30 days (stage of the formation of horny layer and the proliferation of epidermis), SH-group by i-N. P, method and SS-group by DDD method show the strongest colour reaction.
Stage VI : Later than 30 days after stripping (convalescent stage), all colour reactions are restored to normal.
2. Accordingly, it is considered that the increase in SH-group by d-N. P. method indicates the preventive reaction and the same by i-N. P. and DDD methods is related to the cornification, while the increase in SS-group by DDD method suggests the formation of horny layer.
