Abstract
1. Elevated blood fibrinolytic activity was observed immediately after tonsilectomy, especially in those cases that had excessive hemorrhage or prolonged operation time, tonsil massage, and X-ray, while blood fibrinolytic activity in acute tonsillitis was retained in the normal level regardless of the clinical manifestations and courses
2. Extraction method of fibrinolytic substances from tonsil tissue was improved with use of French Press method and the time and procedures for the extraction were shortened and simplified. Amount of plasminogen activator extracted from tonsil of chronic hypertrophy was not markedly different from that of chronic tonsillitis.
3. Intracellular localization of fibrinolytic substances of tonsilla palatina was investigated by cell fractionation method. Plasminogen activator was present prominently in the soluble, mitochondrial, and lysosomal fractions. Plasmin-like substance was found in the soluble and microsomal fractions. Anti-plasmin substances existed only in the large granule fraction
4. Plasminogen activator extracted from tonsil was markedly inhibited by anti-plasmin agents such as t-AMCHA (Trnsamine) and ε-ACA (Ipsilon)
5. Uptake of 3H-labeled t-AMCHA by tonsil slices was rappid and labeled t-AMCHA was found much in the soluble and mitochondrial fractions.
