Abstract
The effects of S-adenosyl-L-methionine (SAMe), D-methionine and L-methionine on the rat erythrocytes, isolated rat hepatocytes, dipalmitoyl phosphatidyl choline (DPPC) -liposomes and surface tension were studied at various concentrations. The inhibition of SAMe on the hypotonic hemolysis in rat erythrocytes was observed at a concentration of 1×10-3M to 1×10-2M.D-Methionine and L-methionine caused an acceleration on the hypotonic hemolysis. In rat isolated hepatocytes, SAMe caused a decrease of GOT and GPT leakage above 2×10-3M. Phase transition temperture of DPPC-liposomes was not changed by the various concentrations of SAMe, D-methionine and L-methionine. SAMe and D-methionine indicated a slight depression of surface tension at a concentration of 1×10-3M. SAMe showed the stabilizing effects on the membrane of rat erythrocytes and hepatocytes, but had no effect on the DPPC-liposomes. The significant membrane stabilizing action of SAMe was not related to the surfactant activity.
