Abstract
Anticancer effects was studied in a cell line (OHR) established from human non-a-fetoprotein (AFP) producing hepatoblastoma. In vitro tests for sensitivity were examined by MTT assay, 3H-Thymidine (TdR) uptake test, and Human Tumor Clonogenic Assay (HTCA) . The culture cell line were dosed with Adriamycin (ADM), Vincristine (VCR), Mitomycin-C (MMC), Cisplatin (CDDP) and 5-fluorouracil (5FU) . The results of each test for sensitivity were: 1) With the MTT assay, all the agents were dose-dependent. ADM, VCR, MMC and 5FU were time-dependent as well, but CDDP was not. The Cytotoxicity Index of ADM, VCR and 5FU were 60 % or more. 2) In the 3H-TdR uptake test, all agents were dose-dependent. The cytostatic effect of ADM was particularly high with low concentration. The Inhibition Index of ADM, MMC and 5FU were 60% or more. 3) The inhibiting rates of colony-formation of ADM and 5FU were less than 60 %, and no inhibiting effect was found in CDDP. This study demonstrated that ADM, VCR and 5FU have cytostatic effects for this cell line.
