Abstract
A clathrate of cycloartenol ferulic acid ester (CAF) with 19-cyclodextrin (β-CyD) has been developed to improve the intestinal absorption of CAF, the major component of γ-oryzanol which is clinically used for autonomic imbalance. We studied the effect of CAF-β-CyD in rat fed with high cholesterol diet (HCD) and compared the result with that of CAF. Male SD rats (130-150 g) received 300 mg/kg CAF, 180, 600, or 1800 mg/kg CAF-β-CyD or 1500 mg/kg β-CyD once a day for 12 days during HCD feeding. Blood levels of total cholesterol (TC), phospholipids (PL), free cholesterol (PC), and triglyceride (TG) were examined on days 6 and 12. In animals fed HCD only, serum TC, PL, PC, and TG were markedly increased on both days 6 and 12, whereas cholesterol in high density lipoprotein (HDL-C) tended to decrease on day 6 but increase on day 12. Administ-ration of 600 or 1800 mg/kg CAF-β-CyD inhibited inreased TC, FC, PL, and TG, and decreased HDL-C. Administration of CAF alone inhibited the increases of TC and PL. The antihyperlipidemic action of CAF-β-CyD tended to appear from 600 mg/kg, which is equivalent to 100 mg/kg CAF : this suggests that CAF-β-CyD is more potent than CAF. Also, CAF-β-CyD seemed to have an effect on lipid metabolism in the liver ; antagonizing the decrease in PL induced by HCD feeding.
