Abstract
Glioma remains one of the most deadly cancers, for which efficacy of traditional chemotherapy is often limited due to the blood-tumor barrier (BTB). Thus, the method for enhancing the BTB permeability is highly needed. In this study, we used a photomechanical wave (PMW) that is generated by irradiation of a light-absorbing material with a nanosecond laser pulse to enhance the BTB permeability in a rat C6 glioma model. A tumor was grown in both hemispheres in a rat, and an Evans blue (EB) solution, as a test drug, was injected into the tail vein. A PMW was applied to the right hemisphere through the cranial window. We compared intensity distributions of EB fluorescence in the three regions of interest (ROIs): tumor core and its adjacent upper and lower regions, between the ipsilateral and the contralateral hemisphere (n=5). Averaged fluorescence intensities in the tumor core and its adjacent upper region of the ipsilateral hemisphere were significantly higher than those of the contralateral hemisphere (paired t-test), indicating efficacy of PMW application for increasing the BTB permeability.
