Abstract
Numerous studies showed the central role of hippocampal dentate gyrus for the pattern separation. However, little is known about the influence range of spatial pattern input and the size of cell assembly performing the pattern separation. Here, we demonstrated the distance dependency of synaptic plasticity induced by tetanic stimulation. We developed a novel culturing device on a patterned ITO coated glass and cultured dissociated hippocampal cells on it. The stimulation-evoked response was observed using calcium imaging. Moreover, we found c-fos expression level was correlated with the spatial location, suggesting the distance dependency of stimulation-induced synaptic plasticity.
