二系統流路を有する密閉型三次元細胞培養システムの開発

抄録

The three-dimensional cell culture is the powerful tool for evaluate behavior of virus and medicine in vitro compared with two-dimensional dishes. In study we developed two lines perfusion culture systems with three-dimensional culture vessel in which one line was for culture cell and another one was for delivering virus or medicine. The three-dimensional culture vessel is composed of three-dimensional fibrous scaffold which is manufactured by laminating microfibers. This system constructed with closed circuit, silicone tube, roller pump, reservoir and three-dimensional scaffold. All devices except pump were set on CO2 incubator. For evaluating culture condition, we measured continuously pH and temperature change in 96 hours run. As a result, two system perfusion culture systems were able to maintain environment suitable for a cell culture at temperature and pH in 96 hours run.