All biological functions, including vision, are affected by time of day. It is known that the retinal response to visual stimuli changes according to circadian rhythms. However, the effects on visually guided behavior were poorly understood, and previous results have been inconsistent. The previous studies examined the effect of time on contrast sensitivity were also inconsistent, one reason for this could be the order effects, such as fatigue due to long measurement times. To overcome this issue, we applied a tablet PC based measurement system which made the measuring contrast sensitivity quick and easy. Observers participated in the experiment every morning, noon, evening, and night, and performed a task to detect a Gabor patch. The results showed that sensitivity improved from morning to night, and this trend was more pronounced in the mid- to high-frequency band. The improvement was reproduced even when measurements were taken once daily at random times. These results suggest that the improvement was not due to intraday learning or fatigue. We confirmed that our experimental system is useful for measuring the diurnal variation in contrast sensitivity, as it is quick and easy to use.