2020 Volume Annual58 Issue Abstract Pages 222
We elucidated the molecular mechanism of angiogenesis effects of non-contact compression force based on phased-array ultrasonic technologies. Endothelial cells cultured on type I-A collagen gel were induced to differentiate and form tube-like structures concomitant with the cyclic compression at apical cell-surface. Additionally, cyclic compression induced high-frequency Ca2+ oscillations which were reduced with the removal of compression. Cyclic compression by our device accelerated wound healing in an acute murine wound model. Compression-stimulated wounds demonstrated significantly accelerated wound healing: their mean area decreased significantly faster relative to control wounds. The histochemical staining indicated the facilitation of collagen proliferation and proangiogenesis in the treatment group. Gene expression profiling revealed similarities between the in vitro and in vivo. Notch ligand and Notch signaling that were involved in angiogenesis were sequentially and transiently upregulated in the early stage after starting treatment. Compression may stimulate to accelerate early angiogenesis mediated by Notch ligand upregulation.
