Urinary analysis of biomarkers in endometriosis
2020 年 Annual58 巻 Abstract 号 p. 236
詳細
2020 年 Annual58 巻 Abstract 号 p. 236
Purpose
Endometriosis is one of most common gynecologic disease, and it can cause dysmenorrhea, infertility, and even malignant transformation that affect patient's life quality. The most common biomarker of endometriosis is serum CA-125 level. There were much increasing articles already presented that inflammatory factors including IL-1, IL-6, IL-8, and TNF-alpha as well as adhesion or angiogenesis factors involving ICAM-1, MMPs, TIMPs, and VEGF can be utilized. However, those studies usually spend much times with intervention approaches. Urine is the easiest collected specimen without invasion. Therefore, we want to developed quick test of endometriosis based on urine specimens.
Methods
In our present study, we also collected urine from patients of endometriosis to performed associate proteomics study to search a representative urinary biomarker as a non-invasive detection of endometriosis in our first step study. Then based on above results and our lab's previous experience, a paper-based quick examination test will be developed in our second step study. Besides, a further clinical trial to apply the paper-based test into patients with endometriosis will also be started in our third step study.
Results
In our first step of project, urine analysis started after collection of total 40 patients with clinical endometriosis including endometrioma or adenomyosis, and another 40 persons without endometriosis as controlled group. ELISA test with kit of CA-125, alpha1-antitrypsin (A1AT), Enolase-1, and vitamin D binding protein (VDBP) were used for collected urine specimen. Then much greater amount of alpha1- antitrypsin (A1AT), Enolase-1, and vitamin D binding protein (VDBP) can be detected in endometriosis group than controlled groups. The area under the curve was 0.815 (95% confidence interval 0.707 to 0.933) when evaluated with serum CA-125 combination.
Conclusion
Based on above results, further paper-based microfluidic quick test will be developed in our next step of project. Then further clinical trial will be started with this quick test in patients with endometriosis under different treatment status to validation of the clinical applications in the future.
